Senior Scientist, Molecular Analytics

At Johnson & Johnson, we believe health is everything. Our strength in healthcare innovation empowers us to build a world where complex diseases are prevented, treated, and cured, where treatments are smarter and less invasive, and solutions are personal. Through our expertise in Innovative Medicine and MedTech, we are uniquely positioned to innovate across the full spectrum of healthcare solutions today to deliver the breakthroughs of tomorrow, and profoundly impact health for humanity. Learn more at https://www.jnj.com

Job Function:
Discovery & Pre-Clinical/Clinical Development

Job Sub Function:
Biotherapeutics R&D

Job Category:
Scientific/Technology

All Job Posting Locations:
Spring House, Pennsylvania, United States of America

Job Description:

About Innovative Medicine

Our expertise in Innovative Medicine is informed and inspired by patients, whose insights fuel our science-based advancements. Visionaries like you work on teams that save lives by developing the medicines of tomorrow.

Join us in developing treatments, finding cures, and pioneering the path from lab to life while championing patients every step of the way.

Learn more at https://www.jnj.com/innovative-medicine

We are searching for the best talent for a Senior Scientist, Molecular Analytics to be located in Spring House, PA.

Purpose: We are seeking a motivated and high performing Senior Scientist to join the Cell and Genetic Medicines group within Therapeutics Discovery (TD) to support the development of CAR T cell therapy programs. In this role, you will perform molecular assays to evaluate both viral and non-viral engineered CAR T cells at the genomic and transcriptomic levels. The successful candidate will have experience with advanced analytical skills to assess the efficiency and fidelity of targeted gene delivery along with quantification of various RNA species. Success in this position will require both the ability to independently complete lab responsibilities and to effectively collaborate within a cross-functional team. This role is an exciting opportunity to join a diverse and ambitious discovery team and to work with innovative technology to create life-changing therapies for patients.

You will be responsible for:

• Developing and performing analytical assays to evaluate genomic outcomes following transgene delivery to primary human T cells.
• Leading projects to characterize and quantify various RNA species from transgenic and endogenous genetic elements.
• Advancing projects by performing molecular biology techniques such as DNA/RNA extraction and quantification, qPCR, ddPCR, and next-generation sequencing (NGS).
• Staying current on the latest scientific and technological advancements in the gene editing field, incorporating new knowledge into ongoing projects, and proposing new areas of investigation.
• Analyzing data, preparing reports, and effectively communicating results to senior leadership, internal partners, and external partners as required.
• Maintaining accurate and detailed experimental procedures, data, and results in an electronic laboratory notebook and ensuring compliance with laboratory protocols and safety procedures.
• Working collaboratively with other team members and cross-functional teams to achieve project goals.

Qualifications / Requirements:

Education:

• A minimum of a Bachelor's degree in Molecular Biology, Biomedical Engineering, or a related field is required. An advanced degree is preferred.

Experience & Skills:

Required:

• At least 4 years of relevant experience in a biopharmaceutical or biotechnology setting is required.
• Background in molecular biology techniques, including DNA/RNA extraction and quantification, qPCR, and ddPCR is required.
• Experience with non-viral targeted integration technologies (which may include, but not limited to CRISPR, site-specific integrases, Prime-editing, etc.) is required.
• Experience with library preparation of NGS platforms such as Illumina short read sequencing, PacBio and/or Oxford Nanopore long read sequencing, RNA-seq, etc. is required.
• Ability to independently perform lab-based responsibilities with limited guidance is required.
• Ability to collaborate with others to plan experiments and coordinate frequent data packages to members of other teams is required.
• Self-motivated with excellent organizational and time management skills is required.

Preferred:

• Molecular cloning experience is preferred.
• Primary human T cell culture, expansion, and genetic engineering (viral transduction and/or non-viral gene editing) experience is preferred.

Johnson & Johnson is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, age, national origin, disability, protected veteran status or other characteristics protected by federal, state or local law. We actively seek qualified candidates who are protected veterans and individuals with disabilities as defined under VEVRAA and Section 503 of the Rehabilitation Act.

Johnson and Johnson is committed to providing an interview process that is inclusive of our applicants' needs. If you are an individual with a disability and would like to request an accommodation, please email the Employee Health Support Center (ra-employeehealthsup@its.jnj.com) or contact AskGS to be directed to your accommodation resource.